Pacini may refer to the following persons:

• Piero Pacini da Pescia (flourished 1495-1514), Italian publisher
• Giovanni Pacini, a 19th-century Italian composer, known mostly for his operas
• Sante Pacini (1735 - circa 1790), Italian painter and engraver active mostly in Tuscany in an early Neoclassic style

• Alfredo Pacini, an Italian prelate of the Roman Catholic Church
• Domenico Pacini, an Italian physicist
• Edoardo Pacini, an Italian footballer
• Émilien Pacini, a 19th-century French librettist of Italian origin
• Filippo Pacini, an Italian anatomist
• Francesco Pacini (born 1906, date of death unknown), Italian modern pentathlete
• Francesco Pacini (footballer)
• Franco Pacini, an Italian astrophysicist
• Giuseppe Pacini, an Italian footballer
• José Pacini, an Argentine football player
• Mark Pacini, an American video game designer
• Regina Pacini, a Portuguese lyric soprano
• Roberto Pacini, an Italian director, author and theatre and film producer
• Sophie Pacini (born 1991), German-Italian pianist

It may also refer to:

• Pacinian corpuscle, mechanoreceptor responsible for sensitivity to deep pressure touch and high frequency vibration